Composite

Part:BBa_I716177

Designed by: Kristin Doan   Group: iGEM07_Berkeley_UC   (2007-08-13)


[RBS][hemA from CFT073][RBS][hemB][RBS][hemD]

Single RBS 1106A + hemA from CFDT073 + RBS + hemB + RBS + hemD

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1834
    Illegal PstI site found at 3125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal NheI site found at 52
    Illegal PstI site found at 1834
    Illegal PstI site found at 3125
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 3110
    Illegal EcoRI site found at 1
    Illegal BglII site found at 59
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1834
    Illegal PstI site found at 3125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1834
    Illegal PstI site found at 3125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4212


[edit]
Categories
Parameters
None